Description

Coupled with user-supplied primer sets and template, PowerUp™ SYBR™ Green Master Mix is designed to amplify targets for accurate gene expression analysis.

A dual hot-start mechanism for excellent specificity
Highly reproducible CTs over a broad dynamic range
Inclusion of UDG to help prevent carryover contamination
Stability of pre-assembled reactions for up to 72 hours
Compatibility with most real-time qPCR instruments

Exceptional specificity with dual hot-start mechanism:

Specificity is paramount in obtaining high-quality data in SYBR™ Green reactions and can be enhanced through control of the Taq DNA polymerase at lower temperatures before stringent primer binding
PowerUp™ SYBR™ Green Master Mix uses the Dual-Lock™ Taq DNA polymerase enzyme that combines two unique hot-start mechanisms to help control its activity and to prevent undesirable early activity of the polymerase at low temperatures
In an experiment evaluating 24 different primer sets, PowerUp™ SYBR™ Green Master Mix generated single melt curves 100% of the time, consistent with highly specific amplification

Tight reproducibility in CTs over a wide dynamic range:

PowerUp™ SYBR™ Green Master Mix demonstrates excellent reproducibility over a wide dynamic range for a variety of targets tested
In addition, this master mix provides efficient amplification over 6 logs of sample input
Unlike competitor mixes that can exhibit inhibition at high cDNA inputs and low correlation coefficients over a dynamic range experiment, PowerUp™ SYBR™ Green Master Mix efficiency is maintained over the full dynamic range to help deliver maximal confidence in data quality

UDG included for carryover contamination control:

The inclusion of uracil-DNA glycosylase (UDG) and dUTP in the PowerUp™ SYBR™ Green Master Mix enables the degradation of any previously amplified PCR products, helping prevent contamination of subsequent qPCR reactions and possible false positives

Stability of pre-assembled reactions:

The tight control of the Dual-Lock™ Taq DNA polymerase in PowerUp™ SYBR™ Green Master Mix enables reactions to be assembled up to 72 hours prior to cycling without impacting data
This consistency allows users to have confidence and flexibility in the use of PowerUp™ SYBR™ Green Master Mix across numerous workflow scenarios1

Broad instrument compatibility:

PowerUp™ SYBR™ Green Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems™ real-time PCR instruments2
It is also compatible with the Bio-Rad IQ™5 and CFX96™/CFX384™ systems, Roche LightCycler™ LC480, and Stratagene™ MX3005P™ systems
For optimal results, recommended primer concentrations are 300 to 800nM

Product code:: 15366158

£138.00Inc. VAT

In Stock

This product is considered

Get a Quote

Which option should I choose?

Automatic Quote, ready for your PO. We'll email you a PDF quote ready for your PO with a direct checkout link.

Quote from Our Team. Our sales team will review your enquiry and contact you personally. Best for specific requirements or special pricing requests.

Delivered by our team here at Trafalgar

Description

Coupled with user-supplied primer sets and template, PowerUp™ SYBR™ Green Master Mix is designed to amplify targets for accurate gene expression analysis.

A dual hot-start mechanism for excellent specificity
Highly reproducible CTs over a broad dynamic range
Inclusion of UDG to help prevent carryover contamination
Stability of pre-assembled reactions for up to 72 hours
Compatibility with most real-time qPCR instruments

Exceptional specificity with dual hot-start mechanism:

Specificity is paramount in obtaining high-quality data in SYBR™ Green reactions and can be enhanced through control of the Taq DNA polymerase at lower temperatures before stringent primer binding
PowerUp™ SYBR™ Green Master Mix uses the Dual-Lock™ Taq DNA polymerase enzyme that combines two unique hot-start mechanisms to help control its activity and to prevent undesirable early activity of the polymerase at low temperatures
In an experiment evaluating 24 different primer sets, PowerUp™ SYBR™ Green Master Mix generated single melt curves 100% of the time, consistent with highly specific amplification

Tight reproducibility in CTs over a wide dynamic range:

PowerUp™ SYBR™ Green Master Mix demonstrates excellent reproducibility over a wide dynamic range for a variety of targets tested
In addition, this master mix provides efficient amplification over 6 logs of sample input
Unlike competitor mixes that can exhibit inhibition at high cDNA inputs and low correlation coefficients over a dynamic range experiment, PowerUp™ SYBR™ Green Master Mix efficiency is maintained over the full dynamic range to help deliver maximal confidence in data quality

UDG included for carryover contamination control:

The inclusion of uracil-DNA glycosylase (UDG) and dUTP in the PowerUp™ SYBR™ Green Master Mix enables the degradation of any previously amplified PCR products, helping prevent contamination of subsequent qPCR reactions and possible false positives

Stability of pre-assembled reactions:

The tight control of the Dual-Lock™ Taq DNA polymerase in PowerUp™ SYBR™ Green Master Mix enables reactions to be assembled up to 72 hours prior to cycling without impacting data
This consistency allows users to have confidence and flexibility in the use of PowerUp™ SYBR™ Green Master Mix across numerous workflow scenarios1

Broad instrument compatibility:

PowerUp™ SYBR™ Green Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems™ real-time PCR instruments2
It is also compatible with the Bio-Rad IQ™5 and CFX96™/CFX384™ systems, Roche LightCycler™ LC480, and Stratagene™ MX3005P™ systems
For optimal results, recommended primer concentrations are 300 to 800nM

Product code:: 15366158

£138.00Inc. VAT

In Stock

This product is considered

Get a Quote

Which option should I choose?

Automatic Quote, ready for your PO. We'll email you a PDF quote ready for your PO with a direct checkout link.

Quote from Our Team. Our sales team will review your enquiry and contact you personally. Best for specific requirements or special pricing requests.

Delivered by our team here at Trafalgar

PowerUp™ SYBR™ Green Master Mix for Qpcr Pk 2

PowerUp™ SYBR™ Green Master Mix for Qpcr Pk 2

PowerUp™ SYBR™ Green Master Mix for Qpcr Pk 2

PowerUp™ SYBR™ Green Master Mix for Qpcr Pk 2

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PowerUp™ SYBR™ Green Master Mix for Qpcr Pk 2

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